Application of deuterium oxide (D2O) to metabolic research: just D2O it? Depends just how you D2O it!
نویسندگان
چکیده
Daniel J. Wilkinson, Philip J. Atherton, Bethan E. Phillips, Paul L. Greenhaff, and Kenneth Smith Division of Medical Sciences and Graduate Entry Medicine, Medical Research Council-Arthritis Research United Kingdom Centre of Excellence for Musculoskeletal Ageing Research, Royal Derby Hospital Centre, University of Nottingham, Derby, United Kingdom; and School of Life Sciences, Queens Medical Centre, Nottingham, United Kingdom
منابع مشابه
Thermoluminescence in ultra-high dilution research.
OBJECTIVE Thermoluminescence of homeopathically prepared ultra-high dilutions of lithium chloride in deuterium oxide (D2O) (10 (-30) g cm (-3); C15 LiCl in D2O) and similarly prepared D2O (C15 D2O) was compared in three experiments varying (1) time between preparation of substance and time of experimentation, and (2) time between irradiation and thermoluminescence recording. Data were also comp...
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BACKGROUND Pancreatic cancer constitutes an entity which is difficult to treat and, therefore, mostly fatal. Since heavy water (deuterium oxide, D2O) was shown to be active in various cancer cell lines in vitro and in vivo, we now investigated its effects in human pancreatic tumor cells. MATERIALS AND METHODS The cytotoxic effects of D2O were examined in three pancreatic cancer cell lines (As...
متن کاملAccumulation of deuterium oxide in body fluids after ingestion of D2O-labeled beverages.
A simple low-cost procedure was developed to compare the temporal profiles of deuterium oxide (D2O) accumulation in body fluids after ingestion of D2O-labeled solutions. D2O concentration was measured in plasma and saliva samples taken at various intervals after ingestion of 20 ml of D2O mixed with five solutions differing in carbohydrate and electrolyte concentrations. An infrared spectrometer...
متن کاملInternal comparison between deuterium oxide (D2O) and L-[ring-13C6] phenylalanine for acute measurement of muscle protein synthesis in humans
Stable isotope tracer methodologies are becoming increasingly widespread in metabolic research; yet a number of factors restrict their implementation, such as, i.v infusions, multiple cannulae, tissue samples, and significant cost. We recently validated the sensitivity of the orally administered stable isotope tracer deuterium oxide (D2O) for quantifying day-to-day changes in muscle protein syn...
متن کاملRelation of growth hormone response to growth hormone-releasing hormone to estimation of milk production via deuterium oxide dilution in beef cattle.
Current methods of estimating milk production in beef cows can be time-consuming, labor-intensive, and subject to high variability. The weigh-suckle-weigh (WSW) method requires repeated separation of offspring from their dams. Machine milking requires that animals be acclimated to the equipment prior to the estimation. The objective of Exp. 1 was to validate a deuterium oxide (D2O) dilution met...
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عنوان ژورنال:
- American journal of physiology. Endocrinology and metabolism
دوره 308 9 شماره
صفحات -
تاریخ انتشار 2015